miR-2400在牛各组织中表达及其靶基因预测

The Expression of miR-2400 in Bovine Tissues and Predicted Target Genes

miRNA作为生物体内的单链非编码小RNA,在调节细胞生长、增殖和分化等生物学过程中的作用,使其受到越来越多的关注。miR-2400是牛中特异表达的miRNA,但其在牛各组织中的表达规律及其可能的靶基因尚不清楚。本研究首先介绍了miR-2400在牛基因组中的位置及序列信息,通过茎环荧光定量PCR技术检测了牛各组织中miR-2400的表达,结果表明,其在皮肤中表达最高,其次是结缔组织和脂肪组织;且在增殖状态的牛骨骼肌卫星细胞中的表达高于分化状态时的表达。miRNA主要是通过其"种子序列"与靶基因mRNA的3’UTR结合从而发挥调控作用。为了更好地了解miR-2400的作用及机制,本研究通过在线软件预测了其可能结合的靶基因,发现大约有544个基因mRNA的3’UTR含有miR-2400潜在的结合位点,大部分靶基因参与细胞的增殖、分化和凋亡。本研究结果为进一步深入研究miR-2400在牛各组织中的作用奠定基础。

MicroRNAs are a family of non-coding small RNAs,approximately 22 nucleotides in length,have been shown to play critical roles in the regulation of muscle cell proliferation and differentiation.MicroRNA-2400,one of miRNA,is a novel and unique miRNA from bovine.However,the expression pattern for miR-2400 in bovine tissues and target genes which miR-2400 targeted has not been reported.In the present study,the position and sequence information of miR-2400 in bovine genome were reported,then the expression of miR-2400 in bovine tissues were detected by stem-loop real-time PCR.The results showed that the expression levels of miR-2400 were highest in skin,followed by connective tissue and adipose tissue.And the expression levels of miR-2400 were higher in proliferated skeletal muscle-derived satellite cell than in differentiated cells.MicroRNAs mainly repress the translation and accelerate the decay of m RNAs through pairing of their seed sequences with the 3’UTRs of target genes.To further investigate the mechanism of miR-2400,the genes that miR-2400 targeted were predicted by bioinformatics prediction(TargetScan and PicTar).There are 544 target genes which 3’UTR contained a conserved binding site for the seed sequence of miR-2400,and most of the target genes are involved in cell proliferation,differentiation and apoptosis.This study provides a basis for further study of the role of miR-2400 in bovine organizations.