基于高选择性SERS传感器的过氧亚硝基阴离子检测

Detection of Peroxynitrite Anion Based on Highly Selective SERS Sensors

过氧亚硝基阴离子(ONOO^(-))是一种重要的活性氮/氧物种,具有极强的氧化和硝化能力,能与生物体内的硫醇、蛋白质、脂质和DNA等多种生物分子发生反应,产生一系列有害的生化效应,如氧化应激和炎症等,导致细胞死亡和器官损伤。因此,检测生物体内的ONOO^(-)对于疾病的早期诊断具有重要意义。基于此,我们利用3-甲氧基苯硼酸频那醇酯(3-MAPE)修饰金纳米颗粒(Au NPs)制备了高选择性传感ONOO^(-)的表面增强拉曼光谱传感器(Au NPs/3-MAPE)。该传感器的原理是ONOO^(-)可以将探针上的硼酸基团氧化成羟基,从而引发传感器的SERS光谱改变。结果表明,该传感器对ONOO^(-)的选择性高,具有优异的灵敏度和检测限,检测限低至0.48μmol·L^(-1)。该传感器可以满足对ONOO^(-)的检测需求,具有很高的应用潜力。

Peroxynitrite anion(ONOO^(-))is an important reactive nitrogen species/oxygen species that can cause oxidative stress and inflammation in organisms by reacting with various biological molecules,including proteins,lipids,and DNA.The detection of ONOO^(-)in organisms is crucial for the early diagnosis of diseases.To address this,we developed a highly selective surfaceenhanced Raman spectroscopy sensor for ONOO^(-)by modifying gold nanoparticles with 3-methoxyphenylboronic acid pinacol ester(Au NPs/3-MAPE).The principle of the sensor is based on the oxidation of boric acid groups on the probe by ONOO^(-)to hydroxyl groups,which triggers changes in the SERS spectrum of the sensor.Our results indicate that the sensor has high selectivity for ONOO^(-),excellent sensitivity,and a low detection limit of 0.48μmol·L^(-1).Therefore,the sensor has great potential for the detection of ONOO^(-)and can be used in various applications.