基于藻红B为光散射探针的共振瑞利散射法快速检测手性药物麻黄碱

Rapid Detection of Chiral Drug Ephedrine by the Resonance Rayleigh Scattering Method Using Erythrosin B for Light Scattering Probe

麻黄碱和伪麻黄碱是临床上常见的手性对映体药物,两者因其手性而药理和疗效有所不同。本实验在Pd2+存在时,藻红B-Pd2+反应体系具有较强的共振瑞利散射(resonance Rayleigh scattering,RRS)强度,加入麻黄碱能使体系的IRRS降低,而伪麻黄碱无此现象。据此光谱差异可实现了这两者的手性识别。同时反应体系的RRS强度减弱程度与麻黄碱浓度成正比,在优化条件下,其线性范围为在40~960 ng·mL-1,其检测限为3.9 ng·mL-1。据此可建立快速检测麻黄碱对映体的新方法。以此为基础,可以发展同时测定麻黄碱和伪麻黄碱手性对映体的手性分析的新方法。

Ephedrine and pseudoephedrine which are chiral enantiomers commonly used clinically have different pharmacological actions and treatment effects due to their chiral nature.In the presence of Pd2+,the reaction system of Ery B-Pd2+have a strong RRS intensity.Adding ephedrine into this system would make the RRS intensity reduce,but pseudoephedrine could not produce the phenomenon.The chiral recognition of these enantiomers could be achieved accord to this spectral difference.In the same time,the reduction of RRS strength of the reaction system is proportional to the concentration of ephedrine.Under optimized conditions,the linear range is 40~960 ng·mL-1,and the detection limit is 3.9 ng·mL-1.A new method for rapid detection of ephedrine enantiomers can be established.Based this assay,a new method for the determination of the chiral enantiomers of ephedrine and pseudoephedrine can be developed.