聚合物荧光温度计的制备及其细胞内温度成像研究

Synthesis of Fluorescent Polymer Thermometer and Its Application for Intracellular Temperature Sensing

细胞内温度变化的研究与代谢通路息息相关,因此构建细胞内的温度响应探针研究能量代谢与温度之间的内在关系至关重要。最近我们构建了一系列基于荧光聚合物的分子温度计并应用于细胞内的温度变化研究。利用聚合物的温度刺激-响应性能引起键合于聚合物链上荧光分子的变化,可以高效灵敏的识别细胞内温度并实现其变化研究。同时,利用这种方法还可以进行外界刺激条件下细胞内温度变化成像,考察与温度相关的神经递质及能量物质的变化关系。

Intracellular temperature changing is related to the most important metabolic pathway.Therefore,temperature sensing in living cells could provide tools in unveiling the intrinsic relationship of energy release and biological processes.In this study,several unique types of polymer based fluorescent thermometers have been constructed and applied in intracellular temperature sensing.The fluorescent thermometers exhibited fluorescence"turn-on"response at higher temperature and could be applied in energy transfer and temperature simultaneous sensing and imaging in living cells.