质谱法和荧光光谱法研究多库酯钠与牛血清蛋白相互作用

Interaction of Docusate Sodium With Bovine Serum Albumin Using Mass Spectrometry and Fluorescence Spectroscopy

多库酯钠(DSS)在日常生活中有着多种用途,研究其与蛋白质的相互作用对于阐明DSS在人体内的结合、转运、代谢有着重要作用。运用了电喷雾质谱法和荧光光谱法研究了DSS与牛血清蛋白(BSA)的相互作用。质谱结果显示DSS可以与BSA形成稳定的蛋白质复合物,表明两者发生了相互结合。荧光结果表明DSS可以使BSA的内源荧光发生较强猝灭,进一步证明了DSS与BSA之间发生了相互作用。此外,DSS猝灭BSA内源荧光属于静态猝灭,表明两者之间具有较强的结合力。

In this paper,the interaction between DSS and BSA was investigated by mass spectrometry and fluorescence spectroscopy.The results of fluorescence measurements show that DSS can strongly quench the intrinsic fluorescence of BSA,which proves the interaction between DSS and BSA.Furthermore,it could be found that BSA fluorescence quenching caused by DSS is static quenching mode,indicating DSS has a strong ability to bind with BSA.More importantly,mass spectrometry results suggest that DSS can form stable protein complexes with BSA,indicating that DSS can bind to BSA.