细胞膜上的金纳米束用于肿瘤细胞分析

A Gold Nanobunadles Held on Plasma Membranes for Tumor Cell Analysis

在肿瘤细胞内,p53蛋白通常会发生变异,变异后p53蛋白(MUp53)的异常表达被认为是刺激肿瘤产生的重要因素之一[1],因为它的干扰细胞的正常生长的调控机制。因此了解p53蛋白的信号通路和在细胞内的表达对癌症诊断具有十分重要的意义。随着暗场显微技术(DFM)和等离子体共振瑞利散射(PRRS)光谱技术的发展,生物传感、单颗粒分析、细胞检测等领域越来越多的出现了等离子体纳米粒子的应用。尤其值得关注的是,AuNP可以吸收可见光并转化为热量,可用于光热治疗[2]。该研究设计了一种基于金纳米颗粒的自组装成等离子体纳米束,用于研究肿瘤光热治疗时p53蛋白表达的通路关系(图1)。该体系在细胞表面结合时,DFM成像可以观察到AuNPs的自组装聚集,并可以通过等离子体瑞利散射谱(plasmon resonance Rayleigh scattering,PRRS),针对探针在肿瘤细胞表面的光照治疗从而发现细胞内p53蛋白的变化,为癌症的诊断提供了新的思路。

The p53 is suppressing tumor protein correlated with the cell cycle factors and apoptosis.AuNP is an attractive nanomaterial candidate with resonant absorption and excitation of incident light to induce strong local surface plasmon resonance(LSPR).With the continuous development of dark-field hyperspectral imaging(DFM)and plasmon resonance scattering spectrum(PRRS),the impact of plasmonic nanoparticles has been successfully applied in biosensing,single particle analysis,colorimetric biosensors,and single-cell monitoring.The absorption spectrum of aggregation gold nanoparticles(AuNPs)shifts to near-infrared(NIR)region which can be observed under dark-field microscopy(DFM)and is treated the subsequent with photothermal therapy.In this paper,a self-assembled plasma nanobundle based on gold nanoparticles is designed to study the pathway relationship of p53 protein expression during tumor photothermal therapy(Figure 1).When the system is combined on the cell surface,DFM imaging can observe AuNPs self-assembly aggregation,and can see the light therapy of plasmon resonance Rayleigh scattering(PRRS),so as to find the changes of p53 protein in cells.This research affords a new strategy for studying intracellular p53 analysis through photothermal therapy on the cell surface and finds a potential in-situ treatment against cancer.