摘要
明胶基粘合剂因其良好的生物相容性被广泛的应用于组织工程领域,但其粘合能力仍存在较大提升空间。在本研究中,通过葡聚糖醛与明胶间的席夫碱反应形成一层交联网络,同时加入转谷氨酰胺酶,使其与明胶利用酶促交联反应形成另一层交联网络,通过双交联网络的形成提高了明胶基粘合剂性能。通过小瓶倒置、溶胀、压缩和搭接剪切等测试凝胶性能及粘合剂性能。结果表明,改性后的明胶基粘合剂具有适宜注射使用的凝胶时间,较低的溶胀比,压缩模量最高可达33.6kPa,是未改性明胶的4倍,粘合强度最高可达25.9kPa,是未改性明胶的12倍。明胶基粘合剂的性能得到了提升。
Because of their good biocompatibility,gelatin-based adhesives are widely used in the field of tissue engineering,but their adhesive ability still needs to be improved.In this paper,the oxidized dextran and gelatin formed one cross-linking network through Schiff base reaction,while enzymatic cross-linking reaction between transglutaminase and gelatin formed another cross-linking network.The formation of the double cross-linking network can enhance the bulk strength of gelatin adhesive.The properties of the adhesive were characterized by vial inversion,swelling,compression,lap shear,etc.The modified gelatin-based adhesive’s gelation time was suitable for injection,and the obtained gel showed a lower swelling ratio.The adhesive’s compression modulus was up to 33.6 kPa,which is 4 times that of unmodified gelatin.And the bonding strength of adhesive was up to 25.9 kPa,it is 12 times that of unmodified gelatin.The performance of gelatin-based adhesives has been improved.
作者
李阳
刘新雨
李思迪
赵瑾
侯信
原续波
LI Yang;LIU Xin-yu;LI Si-di;ZHAO Jin;HOU Xin;YUAN Xu-bo(School of Materials Science and Engineering,Tianjin University,Tianjin 300350)
出处
《高分子通报》
CAS
CSCD
北大核心
2020年第10期37-47,共11页
Polymer Bulletin
关键词
明胶
水凝胶粘合剂
转谷氨酰胺酶
葡聚糖醛
双交联网络
Gelatin
Hydrogel adhesive
Transglutaminase
Oxidized dextran
Double crosslinked network