‘黔茶1号’鲜叶挥发性组分及其相对含量差异分析

Analysis of Volatile Components and Relative Content Differences in Fresh Leaves of‘Qiancha 1’

为探明‘黔茶1号’鲜叶挥发性组分及其相对含量差异,分析其挥发物质积累规律。本研究采用顶空固相萃取-气质联用法,分析了‘黔茶1号’1~3月一芽二叶鲜叶中的挥发性物质。结果表明,6次检出化合物中主要包括醇类、酯类、醛类、烃类、酮类及其他类挥发物共计189种,在不同时间均被检出的化合物52种。其中醇类占比最高达到50.79%~72.69%,酯类占比7.33%~31.77%、醛类占比4.02%~12.19%、烃类占比2.34%~10.12%、酮类及其他类占比0.65%~1.70%,从时间上来看,醇类物质含量在逐渐下降,其他挥发物质的含量在逐渐上升,从温度变化上看,温度升高后,各类物质种类增多,相对含量也在增加。芳樟醇、香叶醇、反-氧化芳樟醇、1-辛醇、顺-氧化芳樟醇、橙花醇、苯甲醇、苯乙醇、α-松油醇、柠檬醛为‘黔茶1号’挥发性成分6次检测中含量较高的物质,通过分析‘黔茶1号’鲜叶挥发物组分,对进一步研究茶树挥发物积累规律、响应病虫害及非生物因子胁迫机制提供基础。

In order to investigate the volatile components and their relative content differences in the fresh leaves of‘Qiancha 1’,the accumulation rule of volatile substances was analyzed.In this study,the volatile compounds in the fresh leaves of‘Qiancha 1’from January to March were analyzed by Head-space solid-phase microeon-gascha-romatography-mass-spectrometry.The results showed that 189 kinds of compounds were mainly included alcohols,esters,aldehydes,hydrocarbons,ketones and other volatiles,and 52 kinds of compounds were detected at different times.Among them,alcohols accounted for 50.79%~72.69%,esters 7.33%~31.77%,aldehydes 4.02%~12.19%,hydrocarbons 2.34%~10.12%,ketones and other classes accounted for 0.65%~1.70%.From the perspective of time,the content of alcohols decreased gradually.The content of other volatile substances is gradually increasing.From the temperature change point of view,after the temperature rises,the types of various substances increase,and the relative content also increases.Linalool,Geraniol,trans-Linalool oxide,1-Octanol,cis-Linalool oxide,Nerol,Benzyl alcohol,Phenylethyl Alcohol,.alpha.-Terpineol and Citral were the high content substances in the six tests of volatile components of‘Qiancha 1’.The analysis of volatiles in fresh leaves of‘Qiancha 1’provided the basis for further study on volatiles accumulation rule,response to pests and diseases and abiotic factors stress mechanism of tea tree.