慢性压迫性神经痛大鼠脊髓水平Reelin蛋白表达变化

The expression of spinal Reelin following chronic constriction injury of the sciatic nerve in rats

目的观察慢性坐骨神经压迫性损伤疼痛大鼠脊髓水平Reelin的表达变化情况.方法54只雄性SD大鼠,随机分为假手术组(Sham组,n=12)和坐骨神经慢性压迫性损伤组(CCI组,n=42).各组大鼠均于术前1d、术后1、4、7、10、14、21和28 d测量双侧后足底痛行为学指标,包括机械缩足阈值(PWMT)和热缩足潜伏期(PWTL).逆转录聚合酶联反应(RT-PCR)、免疫印迹法(Western Blot)检测2组术后1、4、7、10、14、21和28 d脊髓背角Reelin mRNA、Reelin蛋白表达量.结果各组大鼠术前PWMT和PWTL差异无统计学意义(P>0.05);坐骨神经结扎后PWMT和PWTL均出现不同程度降低(P<0.01);与Sham组相比,CCI组Reelin mRNA在腰段脊髓背角水平的表达量减少(P<0.05),Reelin蛋白表达量也相应减少(P<0.05).结论慢性压迫性神经痛下大鼠脊髓水平Reelin蛋白的表达呈现下调.

Objective To detect the expression of Reelin in the lumbar spinal cord of a rat model of neuropathic pain induced by chronic constriction injury on sciatic never(CCI).Methods 54 male SD rats were randomly divided into sham operation group(Sham group,n=12)and CCI model group(CCI group,n=42).Paw withdrawal mechanical threshold(PWMT)and paw wthdrawal themal latency(PWTL)were test-ed on day 1 before operation and day 1,4,7,10,14,17,21 and 28 after operation.Westerm blot and RTPCR were used to detect the protein and mRNA level of Reelin in rat spinal dorsal hom.Results Both PWMT and PWTL have no diference in rats between sham group and CCI group before operation.After sciatic never ligation,both PWMT and PWIL were significantly decreased(P<0.01)while sham operation have no effects on PWMT and PWTL(P>0.05).Compared with sham group,the expression of Reelin mRNA Were significantly decreased in CCI rats(P<0.05),so were the expression of Reelin protein(P<0.05).Conclusion Reelin was decreased in CCI rat spinal dorsal hom.