非受体酪氨酸激酶Etk/BMX促进宫颈癌细胞的迁移及侵袭

Non-receptor tyrosine kinase Etk/BMX promotes migration and invasion of cervical cancer cells

目的检测非受体酪氨酸激酶Etk/BMX对宫颈癌HeLa、SiHa及C-33A细胞迁移及侵袭的影响。方法转染BMX过表达质粒载体至C-33A细胞中,获得稳定过表达BMX的C-33A细胞株;转染一对BMX-TALEN敲除质粒载体,获得稳定敲除的HeLa细胞株;转染shRNA BMX干扰载体,获得稳定干扰的SiHa细胞株。利用划痕实验初步检测BMX对宫颈癌HeLa、SiHa及C-33A细胞迁移的影响;利用Transwell实验进一步检测BMX对宫颈癌细胞迁移(不加基质胶)及侵袭(加基质胶)的影响。结果划痕实验结果显示BMX过表达促进宫颈癌C-33A细胞的迁移能力(P<0.05);划痕实验及Transwell实验结果显示BMX敲除和干扰则显著抑制了宫颈癌HeLa及SiHa细胞的迁移及侵袭能力(P<0.05),E-cadherin因子表达上升而N-cadherin表达下降。结论BMX可以促进宫颈癌细胞迁移及侵袭能力。

Objective To detect the effect of non-receptor tyrosine kinase Etk/BMX on migration and invasion of cervical cancer HeLa,SiHa and C-33A cells.Methods BMX-overexpression plasmids were transfected into C-33A cells to get stable C-33A-BMX overexpression clones;BMX-TALEN plasmids were transfected into HeLa cells to get stable knockout clones;shRNA BMX plasmids were transfected into SiHa cells to get stable BMX knockdown clones;wound healing assay was used to detect the effect of BMX on the migration of cervical cancer HeLa,SiHa and C-33A cells;transwell test was used to further detect the effect of BMX on the migration(without matrigel)and invasion(with matrigel).Results The results of wound healing assay showed that BMX overexpression promoted the migration of C-33A cells(P<0.05);The results of wound healing assay and transwell test showed that knockout or knockdown of BMX inhibited the migration and invasion of HeLa and SiHa cells significantly(P<0.05),at the same time,up-regulated expression of E-cadherin and down-regulated expression of N-cadherin were observed in BMX knockout or knockdown group.Conclusions BMX could promote the migration and invasion of cervical cancer cells.