应用反义寡核苷酸沉默LRIG3表达对宫颈鳞癌Hela229细胞凋亡和侵袭力的影响

Study of the effects of antisense oligonucleotide silencing LRIG3 expression on the apoptosis and invasion of Hela229 cells in cervical squamous cell carcinoma

目的探讨具有亮氨酸重复序列免疫球蛋白样结构域3(the leueine-rich repeats and immunoglobulin-like domains 3,LRIG3)反义寡核苷酸(ASODN)对宫颈鳞癌Hela229细胞凋亡和侵袭力的影响.方法利用阳离子脂质体介导法分别用150、200、250μg/ml的LRIG3 ASODN、正义寡核苷酸(SODN)及无关序列寡核苷酸(MSODN)转染Hela229细胞24、48、72h,应用FITC/PI双标记检测各组细胞凋亡情况,Transwell小室检测各组细胞的侵袭力,以常规培养的细胞作空白对照组.结果转染24、48、72h后,ASOND组细胞凋亡率均较空白对照组、SODN组、MSODN组明显降低(P均<0.05).将250μg/ml LRIG3 ASODN转染Hela229细胞72 h后的细胞做Teanswell侵袭实验,ASODN组与空白对照组、SODN组、MSODN组相比,转染组穿膜细胞数明显增多,侵袭能力增强,且差异均有统计学意义(P均<0.001).结论LRIG3 ASODN可促进宫颈鳞癌Hela229细胞侵袭能力增强,抑制宫颈鳞癌Hela229细胞凋亡.

Objective To investigate the effects of antisense oligonucleotide silencing LRIG3 expression on the apoptosis and invasion of Hela229 cells in cervical squamous cell carcinoma.Methods Hela229 cells were transfected with the ASODN,SODN and MSODN through sequential dose of of LRIG3150μg/ml,200μg/ml and 250μg/ml,which mediated by cationic liposomes at doses of at 24 h,48 h and 72 h.The cells apoptosis rate was detected by FITC/PI double labeling.The cells invasion ability was detected by Transwell chamber.A blank control group was also established.Results Compared with the SODN,MSODN and control group,the apoptosis rate of Hela229 cells was significantly decreased after transfection with ASOND at 24,48 and 72 h.The invasion ability and perforating counts of cells were elevated.Conclusion LRIG3 ASODN can promote the invasion ability and inhibit the apoptosis of Hela229 cells in cervical squamous cell carcinoma.