富硒蒜对幽门螺杆菌诱发的沙鼠腺胃慢性胃炎的疗效

Effect of selenium-enriched garlic on chronic gastritis of the glandular stomach of Mongolian Gerbils induced by H.pylori

目的研究富硒蒜对幽门螺杆菌诱发的沙鼠腺胃慢性胃炎的疗效.方法采用幽门螺杆菌TN2菌株,对雄性长爪沙鼠灌胃5次(1次/4 d),诱发雄性长爪沙鼠慢性胃炎模型,灌胃结束后6个月,将沙鼠按整笼分组法分为6组,包括4个剂量富硒蒜组(4.70、1.5、0.47、0.15g·kg^(-1)·d^(-1))、1个抗生素三联化疗阳性对照组和1个空白对照组,连续灌胃给药4周,停药4周后处死.进行一般症状观察和病理学组织检查.结果富硒蒜灌胃等各组动物的一般情况良好,未见异常;幽门螺杆菌灌胃可诱发沙鼠慢性(萎缩性)胃炎(tAG)、轻度上皮异型增生(DYS)和胃上皮内瘤(GIN)病变.空白对照组(n=13)动物77%发生CAG,38.5%伴上皮异型增生或胃上皮内瘤(DYS/GIN);化疗阳性对照组(n=15)动物40%发生CAG(治愈率48%,X^(2)=3.88,P<0.049),26.7%伴DYS/GIN(治愈率31%);富硒蒜各组(n=21~27)动物腺胃CAG的发生率(38.7%~16.7%)和DYS/GIN发生率(14.3%~11.1%)明显下降,治愈率明显上升(CAG为50%~78%;DYS/GIN为62.9%~71.2%),存在剂效关系,差异有统计学意义(X^(2)=9.34~4.86;P=0.002~0.028).结论富硒蒜能够有效治疗幽门螺杆菌诱发的沙鼠CAG,同时阻断DYS/GIN的发生.

Objective To detect the therapeutic efect of selenium.enriched garlic(SeC)on chronic gastritis.Methods Chronic gastitis was induced of the glandular stomach of male Mongolian Gerbils via gastric itillation of H.pylori TN2 strain once every 4 days for 5 consecutive times fllowed by random casification into six groups.Fresh SeG suspension was administrated daily at dosages of 4.70,1.5,0.47,0.15g·kg^(-1)·d^(-1)for four weeks.The gerbils in the positive control group were treated with omeprazole,clarithromyin,and amoicllin for one week.The grbils were klld for pathlogical examination four weeks after SeG-treatment.Results Chronic gastritis(CAG),low-grade dysplasia or gastric intraepithelial neoplasia(DYS/GIN)were observed among 77%and 38.5%of the 13 H.pylori-treated animals in the negative control group,respectively;whereas 40%and 26.7%in the positive control group(n=15),respectively.The incidences of CAG and DYS/GIN in the SeG groups(n=21-27)were reduced dose-dependently,16.7%-38.7%and 11.1%-14.3%for CAG and DYS/GIN,respectively.Conclusion SeG adminisration inhibits the development and progression of CAG induced by H.pylori remarkably.