纳米氧化石墨烯对胶质瘤细胞放射增敏的探讨

Study on Radio-sensitizing Effects of Glioma by Nano Graphene Oxide

目的 探讨纳米氧化石墨烯(nGO)颗粒对U87胶质瘤细胞的放射增敏作用及其机制.方法 阿拉玛蓝法检测低浓度nGO联合放射对U87细胞存活的影响.凋亡实验检测U87细胞周期及其凋亡.结果 低浓度nGO对U87细胞生长无明显抑制,nGO联合放射具有抑制细胞生长作用,其抑制效应具有时间依赖性,低浓度nGO对U87细胞即有放射增敏作用.nGO、单纯放射均可诱导细胞凋亡,nGO联合放射组凋亡率显著高于前两组(P<0.01),nGO作用后,细胞周期发生变化,表现G2/M期增加(P<0.05).结论 nGO对人脑胶质瘤细胞株U87具有放射增敏作用,其机制可能为抑制细胞周期,阻滞细胞于G2/M期,并诱导细胞凋亡.

Objective To investigate the radio-sensitization effect of nano graphene oxide(nGO)on U87 cells and to explore its mechanism.Methods The effects of low concentration nGO combined with radiation on the survival of U87 cells were detected by Alamar Blue,the radio-sensitization of nGO on U87 cells was detected by cloning,and the cell cycle and apoptosis were detected by flow cytometry(FCM).Results Low concentration nGO had no significant inhibition on the growth of U87 cells.Combined with X-ray,the inhibitory effect was time-dependent rather than dose-dependent.Low concentration nGO had a radio-sensitization effect on U87 cells.nGO and radiation could induce apoptosis,and the apoptosis rates were respectively(2.35±1.43)%,(8.14±1.67)%.The apoptosis rate of the nGO combined radiation group was(27.56±1.04)%,and it was significantly higher than the other two groups(P<0.01).After the action of nGO,the cell cycle changes,which are manifested as decreased S phase and increased G2/M phase(P<0.05).Conclusion nGO has a radio-sensitization effect on human glioma cell line U87,and the mechanism may inhibit the repair of sublethal cell damage,block the cell in G2/M phase,and induce apoptosis.