一株单针藻的筛选、鉴定及保藏

Screening, identification and preservation of a microalgae strain

从自然界中分离纯化出6株微藻,经过200 mL柱式光照反应器通气培养对微藻进行筛选,得到一株具有产油能力的优良藻株.实验结果表明,6株藻种油脂含量均在20%以上,生物量均超过1.2 g/L,其中SS6生长最快速,12d进入稳定期,OD689达到12.270 3,细胞干重达1.337g/L,油脂含量达到30.1%,油脂产率达到25.9 mg/(L·d).经18S rDNA鉴定,SS6藻株为Monoraphidium komarkovae.对比研究多种保藏方法,表明SS6在-18℃、20%甘油保护下可保藏1年以上,复苏后其生长和产油能力均能保持保藏前的状态.

Six strains of microalgae are isolated and purified from the nature.Among them,an excellent oil producing strain is obtained through screening and cultivating microalgae strains in a 200 m L column photobioreactor.Experimental results show that the total liquid contents and dry weights of six strains of microalgae all exceed 20% and 1. 2 g·L^(-1),respectively.Among them,the strain SS6 grows the fastest and enters stable phase after 12 days in the photobioreactor.Its OD689,biomass,grease contents and grease productivity reach 12. 270 3,1. 337 g·L^(-1),30. 1% and 25. 9 mg/( L·d),respectively.Phylogenetic analysis by 18S r DNA identifies that SS6 is Monoraphidium komarkovae.By evaluating various preservation methods,it is found that SS6 can be stored for more than one year at-18℃ under the protection by glycerol( 20% v/v).After recovery,its abilities in growth and oil production both can maintain the states before preservation.