探讨肿瘤标志物RCAS1在肿瘤患者中的检测意义

To explore the detecting value of RCAS1 in tumour patients

目的检测表达在SiSo细胞上的受体结合肿瘤抗原(receptor-binding cancer antigen expressed on SiSo cells,RCASl)在不同肿瘤患者血清及组织中的表达水平,探讨其在肿瘤发生、发展中的作用机制.方法收集肿瘤患者85例(患者组)和健康体检者(对照组)98例,采用免疫组织化学染色方法观察肿瘤组织中RCAS1的表达情况,采用ELISA方法检测受检者血清中RCAS1水平,并对检测结果进行统计学分析.结果免疫组化结果显示RCAS1在肿瘤组织中呈粗颗粒状黄褐色反应产物.患者组RCAS1水平明显高于对照组,差异有统计学意义(P<0.01).鼻咽癌患者组织中RCAS1的阳性表达率为76.2%,且随着病情的加重,血清RCAS1的水平和阳性检出率均不断升高,各组间比较差异均有统计学意义(P<0.01,P<0.05).结论血清RCAS1水平检测可用于肿瘤患者的辅助诊断.

Objective To detet the express levels of recepor-binding cancer antigen expresses on SiSo cells(RCAS1)in serum and tssue,and explore its mechanism on tumor development.Methods 85 ceases patients with tumour(patient group)and 98 cases healthy people(control group)were collected.The expressive condition of RCAS1 in tumour tissue were detected by immunohistochemisty.The levels of serum RCAS1 were detected by ELISA method and the results were analyzed saistically.Results The results of immunohisto chemistry showed that the RCAS1 offered tawny coarse grain reaction product in tumour issue.The levels of serum RCAS1 in patient group were higher than in control group and the difference had statistical significance(P<0.01).The detection rates of RCAS1 in nasopharyngeal cancer were 76.2%.The results showed that the levels and positive detection rates of RCAS1 were increasing along with the aggravation of pathogenetic condition,and the differences among four groups all had statistical significance(P<0.01,P<0.05).Conclusion The serum RCAS1 detecting can be used in auxiliary diagnosis of tumour.