Cross-linking porcine peritoneum by oxidized konjac glucomannan:a novel method to improve the properties of cardiovascular substitute material

The use of natural polysaccharide crosslinkers for decellularized matrices is an effective approach to prepare cardiovascular substitute materials.In this research,NalO_(4)was applied to oxidize konjac glucomannan to prepare the polysaccharide crosslinker oxidized konjac glucomannan(OKGM).The as-prepared crosslinker was then used to stabilize collagen-rich decellularized porcine peritoneum(DPP)to construct a cardiovascular substitute material(OKGM-fixed DPP).The results demonstrated that compared with GA-fixed DPP and GNP-fixed DPP,3.75%OKGM[1∶1.5(KGM∶NalO_(4))]-fixed DPP demonstrated suitable mechanical properties,as well as good hemocompatibility,excellent anti-calcification capability,and anti-enzymolysis in vitro.Furthermore,3.75%OKGM[1∶1.5(KGM∶NalO_(4))]-fixed DPP was suitable for vascular endothelial cell adhesion and rapid proliferation,and a single layer of endothelial cells was formed on the fifth day of culture.The in vivo experimental results also showed excellent histocompatibility.The current results demonstrted that OKGM was a novel polysaccharide cross-linking reagent for crosslinking natural tissues featured with rich collagen content,and 3.75%OKGM[1∶1.5(KGM∶NalO_(4))]-fixed DPP was a potential cardiovascular substitute material.