Effective components and signaling pathways of Ranunculi Ternati Radix based on network pharmacology

Objective:This study was designed to find out the active components of Ranunculi Ternati Radix using network pharmacology,and to explore its potential target and pharmacological mechanism.Methods:By the TCMSP database,combined with oral bioavailability(≥30%)analysis and resistance(≥0.18),screening of active ingredients in Ranunculus ternatus Thunb.Retrieve the protein targets of the compounds from the TCMSP database.Associated Proteins and Gene Names were received via UniProt database.The protein interaction network was constructed by applying String database and Cytoscape software.Gene Ontology and Pathway Enrichment Analysis were developed on the basis of DAVID database.Results:10 active compounds including beta-sitosterol,campesterol,Mandenol were selected from Ranunculi Ternati Radix.And it produced its effects on different diseases mainly by regulating targets including PIK3CG,HSP90AA1,BAX and BCL2,which involved signaling pathways containing Pathways in cancer、PI3K-Akt signaling pathway、Hepatitis B、Tuberculosis and so on.Some published papers had confirmed by each other.Meanwhile,this work predics that Ranunculi Ternati Radix had the potential to treat non-alcoholic fatty liver disease.Conclusion:This study preliminarily validated the major targets and pathways of Ranunculi Ternati Radix acting on different diseases,which laid a foundation for further study on its mechanisms.