摘要
Objective: To explore the mechanism of ShouTaiWan's intervention in the treatment of Threatened Abortion based on Network Pharmacology. Methods: The effective active ingredients and targets in ShouTaiWan were obtained by searching in TCMSP database, BATMAN-TCM database and Uniprot database. The target of threatened abortion was obtained by searching for 'threatened abortion' in GeneCards. And the co-target gene of ShouTaiWan and threaten abortion can be obtained through the use of gene mapping in Venn diagram. Then an interaction network "disease-drug-target" can be constructed through a software called Cytoscape. Upload the Co-targeted gene to the STRING database to construct the protein interaction network map (PPI) , which would be screened for the key genes by using R language. Finally, used the R language to analyze gene ontology (GO) enrichment and kyoto encyclopedia of genes and genome (KEGG) pathway enrichment. Result: It is predicted that 21 effective active ingredients for the treatment of threatened abortion and 89 common effective targets are expected. Quercetin, kaempferol, beta-sitosterol, and isorhamnetin were the most effective components. As for the targets, prostaglandin endoperoxidase receptor (PTGS2), progesterone receptor (PGR), prostaglandin endoperoxidase receptor 1 (PTGS1), and Peroxisome proliferator activates receptor gamma (PPARG) were the most effective ones. The core targets in PPI network are Interleukin-6 (IL-6), protein kinase (AKT1), vascular endothelial growth factor A (VEGFA), etc. GO enrichment analysis shows that the activity of cytokines, regulatory functions, nuclear protein receptors and transcription of genes would be affected. KEGG pathway enrichment analysis illustrates that advanced glycation end products-receptor for AGE (AGE-RAGE) pathways are of the highest significance, followed by the fluid shear stress and atherosclerosis pathways. Conclusion: ShouTaiWan can achieve anti-inflammatory, increase vascular permeability,improve endometrial receptivity and other aspects of regulation to treat threatened abortion and embryo implantation through regulating cytokine.
基金
Special research project of Chinese medicine industry(No.201507001-02)
National administration of traditional Chinese medicine(No.1199ws02)
