摘要
Objective:To analyze the active mechanism of ginseng on breast cancer and its target mechanism.Methods:The Chinese medicine system pharmacology technology platform(TCMSP)database was used to screen out potential chemically active substances and related target proteins in ginseng.The genome annotation database platform(Genecards)was used to predict the target targets of breast cancer,and the uniprot database was used to query and Corresponding gene names,with the help of Cytoscape(3.7.2)software,to build a visualization of the"drug-disease-target"network diagram,construct a protein interaction network through the String database platform,and then use the Bioconductor platform and R language GO enrichment Analysis and KEGG enrichment analysis.Results:Through screening,22 effective chemical components of ginseng,15 key chemicals related to breast cancer,and 35 common targets of ginseng-breast cancer were obtained.The core genes of PPI were MAPK8,AR,RELA,and CASP8.,CYP1A1,CASP3,NCOA1,NR3C1,ICAM1,NCOA2,PGR,AKR1C3,CYP1B1,CYP3A4,GSTP1;Obtain 68 GO biological processes including,and 83 KEGG-related signaling pathways involving TNF signaling pathway,toxoplasmosis pathway Influenza A pathway,fluid shear stress and atherosclerosis pathway,prostate cancer pathway,etc.Conclusion:Ginseng can achieve anti-breast cancer effect through multiple ways,which lays the foundation for the future extraction of effective ingredients to treat breast cancer.
基金
Science and Technology Plan Project of Liaoning Province(No.20180551115).
