摘要
Objective:To evaluate immature mosquitocidal properties of Xanthium strumarium plant extracts against Culex mosquitoes at Entomology Laboratory,Maraki Campus,University of Gondar.Methods:The immature mosquitocidal activity of plant extracts was tested by following World Health Organization recommended protocol.Acetone,methanol and water extracts were prepared at 50,100,150,200 and 250 mg/L concentrations and tested against third and fourth instar larvae and pupae of Culex mosquitoes.The mortality rate of immature mosquitoes was recorded after 24,48 and 72 h exposure period continuously.Results:Third instar larvae after 24 h exposure period,maximum mortality of 77.80%was recorded at 250 mg/L concentration of acetone extract.After 48 h and 72 h exposure period,maximum mortality of 88.90%was recorded in acetone extract in all the tested concentration.The maximum mortality of fourth instar larvae was 88.90%in acetone extract at 200 and 250 mg/L concentrations.Pupal mortality was also greater in acetone extract.The percentage of mortality in all the stage of mosquitoes was higher in acetone extract followed by methanol and water extract.Conclusions:The percentage of mortality is associated with concentration of the extracts tested and exposure period.This laboratory study confirmed immature mosquitocidal activity of Xanthium strumarium leaf extracts against Culex mosquitoes.The aqueous leaf extract can be used by applying on small man-made breeding places to prevent adult emergence.
基金
Supported by University of Gondar(UoG/Budget/no 6215/2015).
