摘要
Objective:To determine the effect of hydroxide compounds on the viability of dermatophytic cells.Methods:Two strains(Trichophyton mentagrophytes and Epidermophyton floccosum)of dermatophytes were clinical isolated from an old male patient with tinea corporis at AL-Hussein General Hospital of Karbala Province.Skin scales of fungal lesion were cultured on Sabouraud dextrose agar containing 0.05 g chloramphenicol.The hydroxide compounds were dissolved in melted fungal media to obtain different percentages(0.625%,1.25%,2.5%,5%).Then the fungal growth on solid media containing tested compounds was determined by using colony diameter method and different concentrations of KOH,NaOH,Mg(OH)_(2)and Ca(OH)_(2)were tested against two species of dermatophytes.Experiments were repeated triplicate for statistical analysis and the data were analyzed for SE of each experiment.Results:KOH and NaOH(down to 1.25%)showed the ability to completely inhibit the growth of both strains of dermatophytes(Trichophyton mentagrophytes and Epidermophyton floccosum).Other two hydroxide compounds revealed variable effects on dermatophytes cells.Conclusions:The strong hydroxide compounds showed a harmful effect on fungal structures and functions.In addition to diagnosis,pathogenic fungi like dermatophytes were killed after treating with these hydroxide agents.
基金
Supported by Pioneer Project Department of Research and Development Directorate of the Ministry of High Education and Scientific Research(Grant No.1026).
