Preliminary study on synergistic combinations of raw honey with gentamicin against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa of veterinary origin

Objective:To search for further synergistic combinations of gentamicin and raw honey that might have potential in treating wounds.Methods:The antibacterial activity and synergistic interaction of raw honey and gentamicin was assessed by using agar well diffusion method.Two Gram-negative(Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 2154)bacteria were selected for antibacterial activity assay.The cultures of bacteria were maintained in their appropriate agar slants at 4°C throughout the study and used as stock cultures.Results:Raw honey and gentamicin interacted synergistically to inhibit Escherichia coli and Pseudomonas aeruginosa.Conclusions:These results suggest that combinations of raw honey and gentamicin have therapeutic benefits in prophylaxis of infections caused by multidrug-resistant Gram-negative bacilli.