摘要
Objective:To investigate the antioxidant properties of starfish Luidia maculata.Methods:The crude ethanol extract was partially purified using liquid-liquid partition and size exclusion chromatography.The antioxidant properties of partially purified fractions were determined using the following methods:determination of total phenolic content,DPPH radical scavenging assay,hydrogen peroxide radical scavenging assay and reducing power assay.Results:Among the five fractions,collected fraction 3,4 and 5 exhibited antioxidant potential.Total phenolic content was high(360μg GAE/g)in fraction 3.In DPPH assay,fraction 3 showed the highest IC_(50)value 0.27 mg/mL and hydrogen peroxide scavenging assay showed the highest IC_(50)value of 0.07 mg/mL in fraction 5.Whereas,fraction 3 showed the highest absorbance(0.3)in reducing power assay when compared to fraction 4 and 5.Conclusions:In conclusion,the present study shows the extract of starfish Luidia maculata(F3)possesses good antioxidant activity.
基金
Supported by University Grants Commission G4(1)1011/12 Govt.of India.
