摘要
Objective:To assess the larvicidal and ovicidal potential of the crude hexane,benzene,chloroform,ethyl acetate and methanol solvent extracts from the medicinal plant,Pithecellobium dulce(P.dulce)against filariasis vector mosquito,Culex quinquefasciatus(Cx.quinquefasciatus).Methods:Twenty five early third instar larvae of Cx.quinquefasciatus were exposed to various concentrations and were assayed in the laboratory by using the protocol of W HO(2005).The larval mortality was observed after 24 h of treatment.The ovicidal activity was determined against Cx.quinquefasciatus mosquito eggs to various concentrations ranging from 100-750 mg/L under the laboratory conditions.Results:The methanol extract of the leaves and seed of P.dulce was the most effective against the larvae with LCs0 and LCgo values 164.12 mg/L,214.29 mg/L,289.34 mg/L and 410.18 mg/L being observed after 24 h of exposure.The efficacy of methanol was followed by that of the ethyl acetate,chloroform,benzene and hexane extracts.The mean percent hatchability of the egg rafts were observed after 48 h of treatment.About 100%mortality was observed at 500 mg/L for leaf and 750 mg/L for seed methanol extracts of P.dulce.Conclusions:From the results,it can be concluded that the larvicidal and ovicidal effect of P.dulce against Cx.quinquefasciatus make this plant product promising as an alternative to synthetic insecticide in mosquito control programs.
基金
Supported by the University Grants Commission(UGC)[F.No.39-646/2010(SR)],New Delhi,India.