摘要
Objective:To determine the antioxidant capacities of pollen extract applied at different concentrations on biochemical parameters in brain tissues of rainbow trouts.Methods:parameters in brain tissues of fish treated at various concentrations of the pollen extract(0.5,2.5,5,10,20 and 30 mg/L)for 96 h.The malondialdehyde levels,total antioxidant status,total oxidant status,oxidative stress index and amounts of total free sulfhydryl groups were analyzed in fish brain.Results:The effective concentration of pollen was determined with some biochemical treated compared to control group(P<0.05).The highest level of total antioxidant status(P<0.05)and the lowest value(P<0.05)of the total oxidant status was 10 mg/L concentration of pollen.Oxidative stress index and level of sulfhydryl groups showed lowest values(P<0.05)in 10 mg/L pollen treated group compared with control group.Conclusions:To apply the pollen to fish reduces the detrimental effects and modulates oxidative status via activating antioxidant defense systems at brain tissue.As a result,pollen can be added up to 10 mg/L to the medium of rainbow trout to improve health of fish.
基金
Supported by Scientific Research Project Found of Cumhuriyet University under the Project number(F-380).
