Investigation of stingray spines by Fourier transform infrared spectroscopy analysis to recognize functional groups

Objective:To investigate functional groups of toxic spines in stingray by Fourier transform infrared spectroscopic analysis.Methods:sephen were centrifuged at 6000 r/min for 10 min.The supernatant was collected and preserved separately in methanol,ethanol,chloroform,acetone(1:2)and then soaked in the mentioned solvents for 48 h.Then extracts were filtered and used for Fourier transform infrared spectroscopic analysis.Results:The venom extract of Himantura gerrardi,Himantura imbricata and Pastinachus and random coiled secondary structure.The presence of O-H stretch,C=O stretch,C-H stretch,N-H deformation,O-H deformation and C-O stretch in the sample aligned with standard bovine serum albumin.The influence of functional groups within the molecule was because of the impact of preferred spatial orientation,chemical and physical interaction on the molecule.In conclusion,compared to bovine serum albumin,Himantura imbricata consists of two C=O stretch,are involved in the hydrogen bonding that takes place between the different elements of secondary structure.Conclusions:The results identified that the presence of free amino acids and protein having β-sheet medicine not available for treatment against injuries causing stingray.Therefore,it's the baseline study,to motivate further process and produce effective antibiotics.The venom of poisonous animals has been extensively studied,since standard.