摘要
Objectives:The wide use of antibiotics for acne is the main cause of drug-resistant Cutibacterium acnes.However,the complete genetic sequence reference of C.acnes has not yet been reported.Herein,we report the complete genetic sequence of Cutibacterium acnes type strain ATCC6919.Methods:Whole-genome sequencing of Cutibacterium acnes ATCC6919 was performed using both the Illumina Hiseq4000 and PacBio RSII single-molecule real-time sequencing platforms.The generated sequence reads were de novo assembled using the HGAP3 protocol of single-molecule real-time analysis v.2.3.0 software.The draft genome was annotated and further analyzed by various bioinformatics tools.Results:The total genome length of Cutibacterium acnes ATCC6919 was 2,495,001 bp(60.02%G+C content),with 2,450 coding sequences including 2,358 proteins,45 tRNAs,9 rRNAs,and 1 sRNA.The Comprehensive Antibiotic Resistance Database showed that the gene(thy A)found in ATCC6919 was associated with resistance to aminosalicylic acid.Antimicrobial susceptibility testing showed that this isolate was not sensitive to metronidazole and sulfamethoxazole,but was sensitive to all other tested antimicrobials(erythromycin,azithromycin,clarithromycin,tetracycline,minocycline,trimethoprim,levofloxacin,ceftriaxone,and doxycycline).Conclusion:This study reports the complete genome sequence of Cutibacterium acnes ATCC6919,which provides a valuable reference for comparative analysis based on the genomic characteristics and resistance gene diversity of Cutibacterium acnes.
