化学发光法检测抗-HCV-IgG与荧光定量PCR检测HCV-RNA在丙型肝炎诊断中的临床评价

Clinical Evaluation of-HCV-IgG in the Diagnosis of Hepatitis C by Chemiluminescence Detection of HCV-RNA and Fluorescent Quantitative PCR

目的:探讨化学发光法(CLIA)检测抗-HCV-IgG与荧光定量PCR检测HCV-RNA在丙型肝炎(HCV)诊断中的临床价值。方法对106例HCV待查者血清标本,同时采用CLIA法检测抗-HCV-IgG抗体和荧光定量PCR检测HCV-RNA载量。结果106份标本中HCV-RNA阳性率为27.4%(29/106),抗-HCV-IgG阳性率为25.5%(27/106),符合率为82.8%(24/29),经χ2检验,两种方法的阳性率差异无统计学意义(P>0.05),抗-HCV-IgG阳性检出率随着HCV-RNA病毒载量的增高而升高。结论 CLIA法检测抗-HCV-IgG抗体与荧光定量PCR检测HCV-RNA在丙型肝炎诊断中无显著差异,但两者均存在一定的局限性,联合运用能有效降低单独使用的漏检风险,提高检出率,为临床诊断HCV感染提供可靠性依据。

Objective To investigate the value of hepatitis C diagnosis using Chemoluminescence immunoassay(CLIA)in the detection of anti-HCV-IgG and Real-time fluorescent quantitative PCR(FQ-PCR)in the detection of HCV-RNA.Methods In 106 suspicious clinical serum samples,the anti-HCV-IgG index was detected by CLIA and the HCV-RNA was detected by FQ-PCR.Results The positive rates of HCV-RNA and anti-HCV-IgG were 27.4%(29/106)and 25.5%(27/106)respectively.There were no significant statistical difference between the two methods(P>0.05)and the coincidental rate was 82.8%(24/29).The positive detection rates of anti-HCV-IgG increased with the elevation of HCV-RNA load.Conclusion CLIA was used to detect anti-HCV-IgG and FQ-PCR in the diagnosis of hepatitis C is not significantly different,but both has some limitations,combined with the can effectively reduce the risk of failure detection used alone,to improve the detection rate,to provide reliable basis for clinical diagnosis of HCV infection.