维吾尔族患者腰椎间盘组织中IL-6的含量及临床相关性

The Content and Clinical Correlation of IL-6 in the Lumbar Intervertebral Disc Tissue of Uygur Patients

目的:检测促炎因子白介素-6(IL-6)和抗炎因子白介素-10(IL-10)在人腰椎间盘组织中的表达含量,并探讨其与临床相关性。方法收集30例单节段椎间盘突出症维吾尔族患者及10例非腰椎源性死亡的新鲜尸体的腰椎间盘组织,术前由一名受培训的医师进行详细病史询问,明确病程长短,对术前临床症状均给予JOA(下腰痛JOA评分)评定,术后应用酶联免疫吸附测定法(ELISA)测定椎间盘组织中IL-6和IL-10的含量。结果维吾尔族突出腰椎间盘组织中IL-6含量较对照组高(<0.01),有统计学意义。脱出型腰椎间盘组织中病例组IL-6含量高于突出性(<0.01),有统计学意义。病例组IL-6和患者病程长短呈负相关,IL-6和患者JOA评分呈负相关。结论突出的腰椎间盘组织有分泌炎症因子的功能术前病程长短与患者手术前下腰痛引起的腰椎功能情况变化有相关性,细胞因子是相互依赖、相互影响的,且与临床密切相关。

Objective The expression contents of pro-inflammatory cytokine IL-6 and in the intervertebral disc and its significance and correlation with clinical findings in Uygur people.Methods Specimens of herniated lumbar disc from 30 Uygur cases of disc herniation in single level and other 10 Specimens from corpse not dead from spinal disease.The clinical symptoms in all cases were evaluated by JOA index preparation,and make sure the patient's history,that all done by one doctor.The contents of IL-6 in the intervertebral disc were detected by enzyme-linked immunosorbent assay.Results The expression levels of IL-6 in Uygur people was significantly higher in the patients than in the controls.Patient's history is having negative correlation with IL-6 contents in the intervertebral disc.Contents of IL-6 in the extrusion intervertebral disc were higher in herniation.Patient's history is have correlation with Patient's JOA index,Patient's JOA index have negative correlation with IL-6.Conclusion The herniated lumbar disc have abilities of excrete pro-inflammatory of cytokines.Patient's history is having correlation with Patient's JOA index.Cytokines is not alone,they react to each other,correlation with clinical findings.