泥鳅粉对训练小鼠游泳力竭24h后心肌S100A4蛋白表达的影响

Effects of Raw Loach to Expression of S100A4 in Mycardium of Mice with Swimming Training at 24 Hours after Exhaustive Exercise

目的从心肌S100A4蛋白表达变化探讨生泥鳅干预对游泳训练力竭小鼠心肌的作用。方法24只健康雄性ICR小鼠分为安静组(C)、运动组(T)、泥鳅运动组(N),T组和N组游泳训练3w后进行力竭运动,24h后取材检测血清CK-MB,HE染色光镜下观察心肌组织,免疫组化法检测心肌P53和S100A4蛋白的表达。结果①N组力竭游泳时间显著地长于T组(<0.01),血清CK-MB活性显著地低于T组和C组(<0.05)。②T组的心肌细胞界限模糊、可见心肌纤维断裂和间质水肿,而N组无明显病理表现。③与C组比较,T组心肌P53蛋白的表达显著增高(﹤0.05);S100A4蛋白N组显著增高(﹤0.05)。结论负荷游泳训练并力竭24 h后小鼠的心肌细胞存在损害,生泥鳅干预能上调S100A4表达和减弱P53表达而降低损害。

Objective This study was to observe ef ects of raw loach on myocardium by expression of S100A4.Methods 24 healthy male ICR mouse were divided into control group(C),exercise-trained group(T)and exercise+raw loach group(N).The exhaustive exercise time of mice was recorded after three week exercise-trained.Activity of serum MB isoenzyme of creatine kinase(CK-MB)was detected.The myocardium were observed by microscope after HE taining.The expressions of S100A4 protein were detected with immunohistochemistry.Results①The time of exercise exhaustive were longer than group T(﹤0.01),the activity of CK-MM were lower than group T and C(<0.05)in group N.②There was not obviously tissue damage in group N.③Compared with group C,the expression of P53 protein increased significantly in group T(<0.05)and the expression of S100A4 protein was increased significantly in group N(P<0.05).Conclusion There was myocardial injury in mice with load swimming training at 24 Hours after exhaustive exercise.But raw loach can reduce the damage of myocardia by increased the expression of S100A4 protein.