摘要
目的:比较三种不同检验方法对阴道细菌的阳性检出率。方法采集本院收治的86例阴道疾病患者的阴道分泌物,分别采取细菌培养法、革兰染色法及PCR法检验阴道分泌物,并将检验结果进行对比分析。结果革兰染色法检验总阳性检出率为75%,PCR法检验总阳性检出率为97.06%;PCR法检验结果与细菌培养法无统计学差异性,>0.05;革兰染色法检验结果与细菌培养法有统计学显著性差异,<0.01;PCR法与革兰染色法有统计学明显性差异,<0.05。结论PCR检验法具有较高的阳性检出率,且操作简便快捷,能够满足临床诊断需要,可于阴道细菌临床检验工作中推广应用。
Objective To compare three different test methods of vaginal bacteria positive detection rate.Methods To col ect in our hospital treated 86 cases of vaginal diseases in patients with vaginal secretion,bacteria culture method were taken respectively,gram staining method and PCR method,inspection vaginal secretions,and the test results were analyzed.Results The gram staining test total positive detection rate was 75%,the PCR method test the positive detection rate of 97.06%;PCR test results and the bacterial culture method with no statistical dif erence,>0.05;Gram staining test results and the bacterial culture method was statistically significant difference,<0.01;PCR method and gram staining method was statistical y significant dif erence,<0.05.Conclusion PCR test has high positive detection rate,and easy operation and quick,can meet the needs of the clinical diagnosis and application in clinical inspection work in the vaginal bacteria.
作者
朱顺新
ZHU Shun-xin(Xiangtan Maternal and Child Health-Care Hospital,Xiangtan 411104,Hunan,China)
