摘要
目的:研究用HER-2抗原负载树突状细胞诱导T淋巴细胞对骨肉瘤细胞系U2-OS的杀伤活性。方法用HER-2抗原肽段E75369-377(HER-2组)、PBS(PBS组)分别负载PBMC来源的树突状细胞,与T淋巴细胞共培养7d后检测各组T细胞穿孔素的表达,通过LDH释放法比较两组T细胞对U2-OS的杀伤活性。结果 HER-2组的T细胞表达穿孔素高于PBS组(P<0.01);HER-2组的T细胞对U2-OS的杀伤率高于PBS组(P<0.01)。结论用HER-2抗原肽E75369-377负载树突状细胞诱导T细胞能提高对骨肉瘤细胞系U2-OS的杀伤效率。
Objective To investigate anti-cancer immunity of the T lymphocyte activated by dendritic cells loading of HER-2 antigen against osteosarcoma cell lines U2-OS.Methods Using HER-2 antigen peptide E75369-377(HER-2 group),PBS(PBS group)loaded PBMC-derived dendritic cells respectively,then cultured with T lymphocytes for 7 days.Detected the expression of T cells'perforin,and compared the kil ing activity of T cells in each group to U2-OS cells by LDH release assay.Results T cells in HER-2 group expressed perforin higher than PBS group(P<0.01);The kil ing rate of T cellto U2-OS cells is higher in HER-2 group than in PBS group detected by LDH release assay(P<0.01).Conclusion The U2-OS whole tumor antigen loading DC to stimulate T lymphocytes activation is bet er than synthetic peptide HER-2 antigen.With HER-2 antigen peptide E75369-377 loading dendritic cells can induce T cells to improve the ability of kil ing osteosarcoma celllines U2-OS.
作者
丁利伟
刘林
许铁
DING Li-wei;LIU Lin;XU Tie(The Graduate School of Xuzhou Medical College,Xuzhou 221004,Jiangsu,China)
