血必净对脓毒症大鼠T细胞凋亡和PD-1/PD-L1表达的影响

Effect of Xuebijing Injection on Apoptosis and PD-1/PD-L1 Expression in T Cells of Rats with Sepsis

目的探讨血必净对脓毒症T细胞凋亡和PD-1/PD-L1表达的影响。方法选用SD大鼠60只,采用经典盲肠结扎穿孔术(cecal ligation and puncture, CLP)复制脓毒症模型,设立正常组、假手术组、CLP模型组、CLP模型加血必净组4组,CLP模型加血必净组大鼠于CLP术后腹腔注射血必净。流式细胞仪检测上述4组小鼠术后12h、24h、36h及48h胸腺T细胞凋亡、胸腺T细胞PD-1和单核细胞PD-L1表达。结果胸腺T细胞凋亡、胸腺T细胞PD-1和单核细胞PD-L1表达在正常组与假手术组比较无统计学差异(>0.05),CLP模型组自12h起各时间点脾淋巴细胞凋亡水平、胸腺T细胞PD-1和单核细胞PD-L1表达均较假手术组明显升高(<0.05)。且各时点CLP模型加血必净组脾淋巴细胞凋亡水平、胸腺T细胞PD-1和单核细胞PD-L1表达均较相应时点CLP组水平下降(<0.05)。结论血必净可以降低脓毒症T细胞凋亡及PD-1/PD-L1表达。

Objective To investigate the ef ects of Xuebijing injection on apoptosis of T cells and expression of PD-1/PD-L1 in septic rats.Methods Cecal ligation perforation(CLP)models were made in rat as septic model.60 rats were randomly divided into four groups:(A)normal group(n=6);(B)sham group(n=6),(C)CLP group(n=24);(D)CLP+xuebijing injection group(n=24).A received sham operation and the others underwent Cecal ligation perforation(CLP)operation.D group were accepted xuebijing injection after CLP models.A and B group were sacrificed on 12 hours after operation.Six rats in C and D group were sacrificed on 12h、24h、36h and 48h after operation.The apoptosis of T cells and expression of PD-1/PD-L1 was assessed by flow cytometry.Results There was no significant dif erence between group A and group B on apoptosis of T cells and expression of PD-1/PD-L1(>0.05).The apoptosis of T cells and expression of PD-1/PD-L1 in group C was increased gradual y on 12 hours after operation(<0.05).The apoptosis of T cells and expression of PD-1/PD-L1 were decreased obviously on each time points in group D compared with group C(<0.05).Conclusion Xuebijing injection can down-regulate the apoptosis of T cells and expression of PD-1/PD-L1 in septic rats.