乙型肝炎患者血清抗黑素细胞抗体检测及与白癜风发病相关性的分析

Analysis of Autoantibodies of Melanocytes in Sera of Patients with Hepatitis B

目的检测乙型肝炎患者血清中抗黑素细胞抗体水平,对其与乙型肝炎患者并发白癜风的相关性进行分析。方法收集经过乙肝五项检测的血清标本88份,根据乙肝五项结果模式分为肝炎组(48例)和非肝炎组(40例),肝炎组标本包括HbsAg、HbeAg、HbcAb阳性(大三阳)8例,HbsAg、HbeAb、HbcAb阳性(小三阳)40例,非肝炎组标本包括HbsAb、HbeAb、HbcAb三抗体阳性(2,4,5)16例,HbsAb阳性(2)16例,全阴性(-)8例,采用ELISA法检测抗人黑素细胞抗体水平,将组间及组内标本的抗体滴度水平,抗体阳性率进行比较,并用SPSS13.0软件分析,>0.05为差异不显著,<0.05为差异显著,<0.01为差异非常显著。结果肝炎组大三阳、小三阳抗黑素细胞抗体阳性率分别为87%(7/8)、85%(34/40),非肝炎组2,4,5阳性、2阳性、全阴性标本为0%(0/16)、0%(0/16)、0%(0/8);肝炎组大三阳、小三阳阳性标本抗体OD均值为1.945±0.652、1.834±0.867,非肝炎组标本OD均值为0.166±0.068、0.196±0.058、0.263±0.034,经比较肝炎组与非肝炎组患者在抗体阳性率和抗体滴度水平上差异非常显著(<0.01),肝炎组内及非肝炎组内患者两项指标差异不显著(>0.05)。并发白癜风的乙肝患者高滴度抗黑素细胞抗体阳性率100%。结论大部分乙型肝炎患者血清内存在高滴度的抗黑素细胞抗体,高抗体水平可能和乙肝患者继发白癜风有关。

Objective By detecting the circulating auto-antibodies to melanocytes in patients'serum with hepatitis B,the risk of hepatitis B patients who would get vitiligo was assessed.Methods 88 patients'serum was col ected and its anto-antibodies to melanocytes was detected by ELISA.The samples divide into two groups,one is the hepatitis B infected group,the other is hepatitis B non-infected group.The infected group involve 8 sample about HbsAg,HbeAg and HbcAb positive(The big three),and 40 samples with HbsAg,HbeAb and HbcAb positive(The small three).The non-infected group involve 16 samples with HbsAb,HbeAb and HbcAb positive,and 16 samples which HbsAb positive,and 8 sample with al negative.After that,OD level was detected by microplate reader under the wavelength of 450nm.The date be analyze by the software of SPASS 16.0.if>0.05 means not remarkable relationship,if<0.05 means remarkable relationship,if<0.01 means intensive remarkable relationship.Results In the hepatitis infected group,the average number of OD in'the big three'is 1.945±0.652,the positive rate about the antibodies of melanocytes is 87%.The average number if OD in'the smal three'is 1.834±0.867,the positive rate about the antibodies of melanocytes is 85%.In the hepatitis non-infected group the rate about the antibodies of melanocytes is 0%,and the average number of OD in the HbsAb,HbeAb and HbcAb positive sample is 0.166±0.068,in the HbsAb positive sample is 0.196±0.058,and in the al negative sample is 0.263±0.034.according the analyze of the date,we find that the rate and titer has intense remarkable relationship between the hepatitis infected group and hepatitis non-infected group.Conclusion Most of patients with hepatitis B has high titer antibodies of melanocytes,it may raise the risk of appearance of vitiligo in these patients.