摘要
目的初步探讨外周血Th17细胞亚群水平与婴幼儿呼吸道合胞病毒(Respiratory Syncytial Virus,RSV)感染的关系,为深入研究婴幼儿呼吸道病毒感染的发病机制奠定基础。方法采用淋巴细胞分离液分离外周血单个核细胞(PBMCs),应用流式细胞术(FCM)分别检测经佛波酯(PMA)和离子霉素(Ionomycin)刺激后的成人组(n=8),健康新生儿组(n=7),患病新生儿儿组(n=5)外周血中CD4+T细胞中IL-17的生成情况。结果新生儿外周血中CD4+IL-17+细胞(即Th17细胞)数量相较成人少很多。但是相比同龄对照组,RSV感染新生儿外周血中Th17细胞亚群的表达却有明显增加。结论RSV感染新生儿外周血中Th17细胞的表达水平升高,提示IL-17和Th17细胞亚群的上升表达可能与新生儿呼吸道病毒感染有一定的关系,为婴幼儿呼吸道病毒感染的发病机制及临床治疗提供了一个新的方向。
Objective To preliminary discuss the relationship between Th17 cells and RSV infection of infants in order to do further study of the pathogenesis of RSV infection.Methods We measured IL-17 production in in PMA and ionomycin stimulated peripheral blood CD4 T cells from RSV patients,infant controls,and adult controls using intracellular flow cytometry(FCM).Results IL-17-producing cells were less abundant in the newborn memory T-cellpopulation.In RSV patient peripheral blood,however,we observed a significant increase of Th17 cells over age-matched controls.Conclusion The upregulation of IL-17 and Th17 cells in RSV infected infants prompted that may be the two has some relationship with each other and provided a new direction for the the pathogenesis of RSV infection and clinical treatment.
作者
张志伟
ZHANG Zhi-wei(Hunan Maternal and Child Health Care Center,Changsha 410008,Hunan,China)
