HLA-A抗原在喉癌鳞状细胞癌中的表达及临床意义

Expression of HLA-A Antigen in Laryngeal Squamous Cell Carcinoma and Its Clinical Significance

目的检测人类白细胞抗原-A抗原(HLA-A)在喉鳞状细胞癌中的表达及临床意义。方法采用免疫组化SP法和流式细胞术,观察20例正常喉组织和58例喉鳞状细胞癌组织中HLA-A抗原的表达,并结合患者的临床病理资料对其结果进行分析。结果①HLA-A抗原在后鳞状细胞癌和正常喉组织中的阳性表达率分别为72.4%(42/58),100%(20/20),二者之间差异有统计学意义。(<0.01)。②HLA-A抗原在各级喉鳞状细胞癌中均有表达,且表达的阳性率随喉癌分化程度的下降而下降,在不同病理分级之间的阳性率有显著性差异(<0.05),且与淋巴结转移及临床分期有关(<0.05)。③喉鳞状细胞癌组与正常喉组织组HLA-A抗原的平均荧光指数分别为(±)(4.53±0.85)和(8.24±1.30),两组比较具有显著性差异(<0.01)。结论 HLA-A抗原可能作为喉鳞状细胞癌发生,发展不同时期的一个标志物,并对判断喉鳞状细胞癌的预后有一定的价值,为临床诊断治疗提供一定的理论依据。

Objective To investigate the expression of Human Leukocyte Anrigen-A(HLA-A)in laryngeal squamous cellcarcinoma(LSCC)and its clinical significance.Methods SP immunohistochemical method and flow cytometry method were used to determine the expression of HLA-A in 58 patients with laryngeal squamous cellcarcinoma and 20 patients with normal laryngeal mucosawas.The results were compared.Results 1)HLA-A was found in 72.4%(42/58)and 100%(20/20)of LSCC and normal laryngeal mucosawas,The dif erence was significance(<0.01).2)The positive rate of the expression of HLA-A in LSCC was significantly variable among dif erent pathological degrees(<0.05).Expression of HLA-A in LSCC is significantly correlated with lymph node metastasis(<0.05)and clinical staging(<0.05).3)The average fluorescence index of HLA-A in LSCC and normal laryngeal mucosa were 4.53±0.85 and 8.24±1.30(x±S)respectively(<0.01).Conclusion HLA-A might be as a marker of the genesis and development of LSCC and may be a helpful marker to determine the malignant degree and prognosis of LSCC.