Rta/IgG在珠海地区鼻咽癌诊断中的应用价值

To Evaluate the Clinical Value of Epstein-Barr Virus (EBV)'s Rta/IgG in the Diagnosis of Nasopharyngeal Carcinoma (NPC) Patients in Zhuhai

目的探讨EB病毒的Rta蛋白抗体IgG(Rta/IgG)在珠海地区鼻咽癌(nasopharyngeal carcinoma,NPC)患者的临床诊断价值。方法收集75例病理证实未经治疗的NPC患者血清、100例健康成人血清及肝癌、胃癌、肺癌、淋巴瘤、宫颈癌血清各19例。检测①Rta/IgG在NPC、肝癌、胃癌、肺癌、淋巴瘤、宫颈癌、健康人群的表达水平;②Rta/IgG与鼻咽癌临床分期关系;③Rta/IgG、VCA/IgA、Rta/IgG联合VCA/IgA对鼻咽癌检测率。结果①NPC患者Rta/IgG阳性率远高于健康人水平,具有显著性差异(<0.01);并高于其他癌种的阳性率,具有显著性差异(<0.01)。②Rta/IgG的表达与NPC临床分期无统计学意义(>0.05)。③Rta/IgG、VCA/IgA及VCA/IgA联合Rta/IgG检测NPC的敏感度分别为81.3豫、89.3豫、94.6豫;特异度分别为93豫、84豫、80%。结论①Rta/IgG是NPC早期辅助诊断及筛查的可靠的血清学指标。②Rta/IgG的表达与NPC临床分期无关。③Rta/IgG联合VCA/IgA检测提高了NPC的筛检和诊断灵敏度。

Objective To evaluate the clinical value of Epstein-Barr virus(EBV)Rta/IgG in the diagnosis of nasopharyngeal carcinoma(NPC)patients in Zhuhai.Methods Rta/IgG and VCA/IgA was detected by enzyme-linkedimmunosorbent assay in 75 NPC patients,100 healthy adults and 60patients with dif erent tumor.Results(1)Significant dif erence of Rta/IgG positive rate between NPC group and the healthy control group(<0.01)as well as between NPC group and other carcinoma groups(<0.01).(2)The expression levels of Rta/IgG in NPC patients serum were irrelative to the clinical stage of the tumor(<0.05).(3)The sensitivity rates were 81.3%,88.9%,94.6%for Rta/IgG,VCA/IgA,Rta/IgG or VCA/IgA respectively,while the specificity rates were 94.6%,84%,80%respectively.Conclusion Rta/IgGis a sensitive and specific serologic parameter for NPC diagnosis.but it is not a proper maker for evaluating clinical stage of NPC.The combined detection of Rta/IgG and VCA/IgA has referential value in diagnosing nasopharyngeal carcinoma patients in Zhuhai.