摘要
目的:探讨压力性尿失禁(SUI)患者尿道周围结缔组织中核心蛋白聚糖(DCN)和转化生长因子β1(TGF-β1) mRNA表达水平的改变及其相关性。方法将65例绝经后妇女根据是否存在SUI,分为SUI组(35例)和对照组(30例)。采用半定量反转录聚合酶链反应(RT-PCR)方法检测两组妇女尿道周围结缔组织中DCN和TGF-β1 mRNA的表达水平。结果 SUI组患者DCN及TGF-β1 mRNA表达水平与对照组比较,其差异均有统计学意义,且两者间存在负相关关系。结论 SUI患者尿道周围结缔组织中TGF-β1减少及DCN的升高可能导致该部位胶原蛋白含量的减少,最终导致SUI发生的原因之一。
Objective To observe the relationship between the expression of decorin and TGF-β1 mRNA in periurethral vaginal tissue in patients with stress urinary incontinence(SUI).Methods Transvaginal biopsies were obtained from the periurethral vaginal tissue in 35 SUI and 30 non SUI control postmenopausal women.RT-PCR were used to verify the mRNA level of decorin and TGF-β1.Results The expression of decorin was significantly higher and the expression of TGF-β1 was significantly lower in patients with SUI.The expression of decorin mRNA was highly and negatively related to the expression of TGF-β1 mRNA.Conclusion Our study results suggest that the decrease of TGF-β1 expression and increase of decorin expression,might reduce the quantity of col agen of periurethral vaginal tissue,and which involved in the pathogenesis of SUI.
作者
欧璐
OU Lu(Department of Gynaecology and Obstetrics,Nanhai District People's Hospital Foshan City,Foshan 528200,Guangdong,China)
