摘要
Objective:To study the effect of Buyi prescription combined with immunotherapy on PD-1/PD-L1 expression in peripheral blood T cells in patients with autoimmune hepatitis.Methods:56 patients with autoimmune hepatitis who were treated in our hospital between May 2014 and December 2016 were randomly divided into two groups with 28 patients in each group. The control group received prednisone combined with azathioprine for routine immunotherapy, and the observation group received Buyi prescription combined with prednisone and azathioprine therapy. 12 weeks after treatment, the serum was collected to determine the levels of liver damage marker molecules, liver fibrosis marker molecules and T cell-related cytokines, and the peripheral blood was collected to determine the PD-1 and PD-L1 expression in CD4+T cells. Results:12 weeks after treatment, fluorescence intensity of CD4+PD-1+ and CD4+PD-L1+T cells in the peripheral blood of the observation group were significantly lower than those of the control group;serum ALT,γ-GT, PC-III, C-IV, IFN-γ and IL-17 levels were significantly lower than those of the control group while IL-4 and IL-10 levels were significantly higher than those of the control group.Conclusion:Buyi prescription combined with immunotherapy can reduce the expression of PD-1/PD-L1, adjust the CD4+T cell function, reduce the liver damage, inhibit the liver fibrosis and treat AIH effectively.
