摘要
Objective:To study the effect of hepatitis B virus X protein (HBx) on the expression of proliferation molecules, invasion molecules and angiogenesis molecules in liver cancer cell lines.Methods: Liver cancer cell lines HepG2 were cultured and divided into HBx group and control group that were transfected with pcDNA3.1-HBx plasmid and blank pcDNA3.1 plasmid respectively. 24 h and 48 h after transfection, the mRNA expression of proliferation molecules Survivin, cyclinD1 and c-myc, invasion molecules CD44v6, MT1-MMP, MMP2 and MMP7 as well as angiogenesis molecules VEGF, Ang-1, Ang-2 and FGF-2 in cells were determined.Results:After 24 h and 48 h of transfection, the Survivin, cyclinD1, c-myc, CD44v6, MT1-MMP, MMP2, MMP7, VEGF, Ang-1, Ang-2 and FGF-2 mRNA expression in HBx group of cells were significantly higher than those in control group.Conclusion: HBx can promote the expression of proliferation molecules, invasion molecules and angiogenesis molecules in the liver cancer cell lines.
