Study and analysis of oxidative stress and cell apoptosis in retinal tissue of diabetic model rats after total flavonoids of onion treatment

Objective:To study the effect of total flavonoids of onion treatment on oxidative stress and cell apoptosis in the retinal tissue of diabetic model rats.Methods:Male SD rats were selected as experimental animals and randomly divided into control group, diabetes group, low-dosage total flavonoids group (total flavonoids L group) and high-dose total flavonoids (total flavonoids H group), intraperitoneal injection of streptozotocin was adopted to make diabetes model, and the total flavonoids L group and H group received lavage of 100 mg/kg and 200 mg/kg total flavonoids of onion for intervention. After 8 weeks of intervention, retinal tissue was collected and the degree of oxidative stress and cell apoptosis were determined.Results:P2X7, ROS, -OH, O2-, H2O2, FADD, p-JNK, caspase-8 and caspase-3 levels in retinal tissue of diabetes group were significantly higher than those of control group while Mn-SOD, CAT, GSH, AsA, NGF, BDNF and CNTF levels were significantly lower than those of control group;P2X7, ROS, -OH, O2-, H2O2, FADD, p-JNK, caspase-8 and caspase-3 levels in retinal tissue of total flavonoids L group and total flavonoids H group were significantly lower than those of diabetes group while Mn-SOD, CAT, GSH, AsA, NGF, BDNF and CNTF levels were significantly higher than those of diabetes group;P2X7, ROS, -OH, O2-, H2O2, FADD, p-JNK, caspase-8 and caspase-3 levels in retinal tissue of total flavonoids H group were significantly lower than those of total flavonoids L group while Mn-SOD, CAT, GSH, AsA, NGF, BDNF and CNTF levels were significantly higher than those of total flavonoids L group.Conclusion:Total flavonoids of onion have inhibiting effect on the oxidative stress and cell apoptosis in retinal tissue of diabetic model rats.