摘要
Objective:To study the correlation of Twist and YB-1 gene expression in cervical cancer and precancerous tissue with cell invasion.Methods:Cervical cancer tissue, precancerous tissue and normal cervical tissue surgically removed in our hospital between May 2013 and April 2015 were collected;immunohistochemical staining kits were used to detect the positive protein expression rate of Twist and YB-1 gene;fluorescence quantitative PCR kits were used to detect Twist, YB-1 and invasion gene mRNA expression.Results:Twist and YB-1 mRNA expression and positive protein expression rate as well as USP22, Rab11, Rac1 and ANXA5 mRNA expression in cervical cancer tissue and precancerous tissue were significantly higher than those in normal cervical tissue, Twist and YB-1 mRNA expression and positive protein expression rate as well as USP22, Rab11, Rac1 and ANXA5 mRNA expression in cervical cancer tissue were significantly higher than those in precancerous tissue;USP22, Rab11, Rac1 and ANXA5 mRNA expression in cervical cancer tissue and precancerous tissue with positive Twist and YB-1 expression were significantly higher than those in cervical cancer tissue and precancerous tissue with negative Twist and YB-1 expression.Conclusion:Highly expressed Twist and YB-1 in cervical cancer and precancerous tissue can promote cell invasion.
