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猪瘟病毒实时荧光RT-PCR检测方法建立应用

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摘要 本文主要探讨了猪瘟病毒实时荧光定量PCR检测方法,该方法采用提取样品中的总RNA,以其中的mRNA作为模板,采用Oligo(dT)或随机引物利用逆转录酶反转录成cDNA,再以cDNA为模板进行PCR扩增,而获得目的基因或检测基因表达.结果表明,本文所提出的CSFV实时荧光定量PCR检测方法具有良好的特异性,精密度和准确性,在对猪瘟病毒进行监测的过程中,检测快速且适用性十分良好.
作者 徐国翔
出处 《兽医导刊》 2019年第12期213-213,共1页 Veterinary Orientation
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