精液中沉默信息调节因子相关酶1、血管内皮生长因子表达水平与男性不育患者生精功能障碍的相关性分析

Correlation analysis of expression levels of silent information regulator associated enzyme 1 and vascular endothelial growth factor in semen and spermatogenic dysfunction in male infertility patients

目的分析研究精液中沉默信息调节因子相关酶1(SIRT1)、血管内皮生长因子(VEGF)表达水平与男性不育患者生精功能障碍的相关性。方法选取2019年3月至2022年12月商丘市第一人民医院收治的162例生精功能障碍的男性不育患者为研究对象,将其分为无精子症组(n=79)与弱精子症组(n=83),另选同期在本院体检正常生育的男性为对照组(n=60)。采用Johnsen评分评价三组睾丸的生精功能;比较三组精液中SIRT1、VEGF表达水平;采用Pearson相关性分析精液中SIRT1、VEGF表达水平与Johnsen评分、精液参数的关系。结果三组的Johnsen评分比较,无精子症组<弱精子症组<对照组,差异均具有统计学意义(P<0.05);三组精液中的SIRT1、VEGF表达水平比较,无精子症组<弱精子症组<对照组,差异均具有统计学意义(P<0.05);Pearson相关性分析结果显示,精液中SIRT1、VEGF表达水平与Johnsen评分、精子浓度、前向运动精子百分率均呈正相关(P<0.05)。结论男性不育患者生精功能障碍与精液中SIRT1、VEGF表达水平相关。

Objective To study the relationship between the expression levels of silent information regulator associated enzyme 1(SIRT1)and vascular endothelial growth factor(VEGF)in semen and spermatogenic dysfunction in male infertility patients.Methods A total of 162 male infertility patients with spermatogenic dysfunction admitted to the First People’s Hospital of Shangqiu City from March 2019 to December 2022 were selected as the study objects,and divided into azoospermia group(n=79)and asthenospermia group(n=83),and other males who underwent physical examination in our hospital during the same period were selected as the control group(n=60).Johnsen score was used to evaluate the spermatogenic function of testis in three groups.The expression levels of SIRT1 and VEGF in semen of three groups were compared.Pearson correlation was used to analyze the relationship between the expression levels of SIRT1 and VEGF in semen,Johnsen score and semen parameters.Results The Johnsen scores of the three groups were compared,azoospermia group<asthenospermia group<control group,the differences were statistically significant(P<0.05).The expression levels of SIRT1 and VEGF in semen of three groups were compared,azoospermia group<asthenospermia group<control group,the differences were statistically significant(P<0.05).The results of Pearson correlation analysis showed that the expression levels of SIRT1 and VEGF in semen were positively correlated with Johnsen score,sperm concentration and percentage of forward motile sperm(P<0.05).Conclusion Spermatogenic dysfunction of male infertility patients is related to the expression levels of SIRT1 and VEGF in semen.