细胞分裂周期蛋白6(CDC6)在胰腺导管腺癌中的表达及临床意义

Expression and clinical significance of cytokine cyclin 6(CDC6) in pancreatic ductal adenocarcinoma

目的研究细胞分裂周期蛋白6(CDC6)在胰腺导管腺癌中的表达及其与临床预后的相关性。方法使用基因表达交互分析(GEPIA)数据库分析CDC6的mRNA在胰腺癌组织及癌旁正常组织中的表达水平,并利用Kaplan-Meier分析CDC6与胰腺癌临床预后的相关性。回顾性分析80例胰腺导管腺癌患者的临床病理特征,通过免疫组化学染色法检测CDC6在胰腺癌组织与癌旁正常组织的表达情况,通过Graphpad 8.0软件对数据进行分析,探讨CDC6蛋白高表达的临床意义。结果免疫组化染色结果显示CDC6在胰腺癌组织的表达远高于正常组织(P<0.05),CDC6与胰腺癌患者的年龄、性别和肿瘤分级的相关性均无统计学意义(均P>0.05),但与肿瘤的大小密切相关(P<0.05)。结论CDC6与胰腺癌的临床预后关系密切,在胰腺癌的发展演化中高度表达,并有望成为胰腺癌治疗的靶点和潜在生物标志物。

Objective To investigate the expression of cytokine cyclin 6(CDC6)in pancreatic ductal adenocarcinoma,and its correlation with prognosis.Methods The expression levels of CDC6 mRNA in pancreatic cancer tissues and normal tissues adjacent to the cancer were analyzed using the gene expression interaction analysis(GEPIA)database,and the correlation between CDC6 and the clinical prognosis of pancreatic cancer was analyzed using Kaplan-Meier.The clinicopathological characteristics of 80 patients with pancreatic ductal adenocarcinoma were retrospectively analyzed.The expression of CDC6 in pancreatic cancer tissues and normal tissues adjacent to the cancer was detected by immunohistochemical staining,and the data were analyzed by Graphpad 8.0 software to explore the clinical significance of high CDC6 protein expression.Results Immunohistochemical staining results showed that the expression of CDC6 in pancreatic cancer tissues was much higher than that in normal tissues(P<0.05),and the correlation between CDC6 and age,gender,and tumor grade of pancreatic cancer patients was not statistically significant(all P>0.05),but closely correlated with the size of the tumor(P<0.05).Conclusions CDC6 is closely related to the clinical prognosis of pancreatic cancer and is highly expressed in the developmental evolution of pancreatic cancer.Therefore,CDC6 is expected to be a target and potential biomarker for pancreatic cancer therapy.