不完全射频消融对兔VX2肝癌MMP-9表达的影响

Effect of incomplete radiofrequency ablation on the expression of MMP-9 in the rabbit VX2 model of hepatocellular carcinoma

目的研究不完全射频消融(RFA)治疗对兔VX2肝癌模MMP-9蛋白表达的影响。方法建立新西兰白兔的VX2肝癌模型,将30只兔VX2肝癌模型分为2组,即对照组和实验组,每组15只,对照组只做开关腹,而不进行RFA;实验组进行开腹消融,消融范围为肿瘤组织的75%;在实验组中设立RFA后快速进展亚组,定义为实验结束时肿瘤的倍增率大于对照组。对比其RFA后的VX2肝癌的体积变化、残留VX2肝癌基质金属蛋白酶-9(MMP-9)的表达情况。结果对照组和实验组治疗后肿瘤体积分别为(7862±1304)mm^(3)和(6996±709)mm^(3),肿瘤的倍增率分别为(291±49)和(232±16),差异有统计学意义(P<0.05)。对照组和实验组MMP-9阳性表达率为52.1%和46.3%,差异均有统计学意义(P<0.05);实验组中3只实验兔肿瘤倍增率及MMP-9表达率明显高于实验组,属于RF后快速进展亚组,差异有统计学意义(P<0.05)。结论部分性消融对大多数肝细胞癌具有一定疗效,但对于少数肿瘤可能加速其生长,而MMP-9的过表达可能是促进残留癌快速进展的原因。

Objective To study the effect of incomplete radiofrequency ablation(RFA)on the expression of matrix metalloproteinase-9 protein(MMP-9)in the rabbit VX2 model of hepatocellular carcinoma.Methods The VX2 liver cancer model of New Zealand white rabbits was established.Thirty New Zealand white rabbits were divided into control group(n=15)and treatment group(n=15).The control group was only operated with switching abdomen without RFA.The treatment group was treated with RFA and the ablation range was about 75%of the tumor volume.The rapid progression subgroup after the establishment of RFA in the experimental group was defined as the tumor doubling rate at the end of the experiment was higher than that in the control group.The tumor volume and expression of MMP-9 were compared.Results The tumor volume was(7862±1304)mm^(3) in the control group and(6996±709)mm^(3) in the treatment group,and the tumor multiplication rate was(291±49)and(232±16),respectively,the difference was statistically significant(P<0.05).The expression rate of MMP-9 in the control gourp and the treatment group was 52.1%and 46.3 with significant differences(P<0.05).The tumor multiplication rate and MMP-9 expression rate of rapid progression subgroup were significantly higher than that of the the treatment group,with statistically significant differences(P<0.05).Conclusions Partial ablation has a certain effect on most hepatocellular carcinoma,but it may accelerate the growth of a few tumors,and the overexpression of MMP-9 may contribute to the rapid progression of residual carcinoma.