摘要
免疫电镜技术是抗原抗体反应和电子显微镜相结合的技术,可在亚细胞和超微结构水平对抗原进行定位观察分析。其在肾脏疾病诊断和研究领域的应用包括单克隆免疫球蛋白沉积性肾病、免疫复合物介导的肾小球疾病、肾小球结构研究、肾小管疾病发病机制以及其他肾脏疾病。本文较为全面地展示了免疫电镜技术在肾脏疾病诊断和研究领域的重要作用和发展趋势,以期对今后相关领域的研究有所启发。
Immunoelectron microscopy is a combination of antigen-antibody reaction and electron microscopy,which can be used to observe and analyze antigens at subcellular and ultrastructural level.Its applications in diagnosis and research of renal diseases involve monoclonal immunoglobulin deposition nephropathy,immune complex-mediated glomerular diseases,glomerular structure research,pathogenesis of renal tubular diseases,and other renal diseases.This review comprehensively showed the important role and development trend of immunoelectron microscopy in diagnosis and research of renal diseases,in order to support the future research in related fields.
作者
沈婉君
王田田
尹智炜
谢院生
Wanjun Shen;Tiantian Wang;Zhiwei Yin;Yuansheng Xie(Department of Nephrology,First Medical Center of Chinese PLA General Hospital,Chinese PLA Institute of Nephrology,State Key Laboratory of Kidney Diseases,National Clinical Research Center for Kidney Diseases,Beijing Key Laboratory of Kidney Diseases,Beijing 100853;College of Integrated Chinese and Western Medicine,Hebei Medical University,Shijiazhuang 050017,Hebei Province,China)
出处
《中华肾病研究电子杂志》
2022年第4期219-223,共5页
Chinese Journal of Kidney Disease Investigation（Electronic Edition）
基金
国家自然科学基金面上项目(82174115)
关键词
免疫电镜
肾脏疾病
肾小球
肾小管
Immunoelectron microscopy
Kidney diseases
Glomerulus
Renal tubule
