肝细胞核因子-1α基因突变协同腺瘤样结肠息肉病基因突变对家族性腺瘤性息肉病细胞增殖的影响

Effects of hepatocyte nuclear factor-1α mutation and adenomatous polyposis coli mutation on familial adenomatous polyposis cell proliferation

目的探讨肝细胞核因子-1α(HNF-1α)基因突变协同腺瘤样结肠息肉病基因(APC)突变对家族性腺瘤性息肉病(FAP)细胞增殖的影响。方法利用RNA干扰技术,构建HNF-1α基因突变协同APC突变细胞模型,通过MTT法、克隆形成、划痕试验、细胞迁移、细胞侵袭及致瘤等实验观察HNF-1α基因突变协同APC突变对FAP细胞的增殖能力。结果与单突变组比较,HNF-1α基因突变协同APC突变组FAP细胞具有较强的细胞克隆形成、细胞迁移、细胞侵袭及致瘤性。结论HNF-1α基因协同APC突变促进了FAP细胞增殖。

Objective To explore the effects of hepatocyte nuclear factor-1α(HNF-1α)mutation and adenomatous polyposis coli(APC)mutation on cell proliferation in familial adenomatous polyposis(FAP).MethodsThe cell model ofHNF-1αcombined withAPCmutation was constructed by RNA interference technique.MTT assay,cell clonal formation,wound healing test,cell migration,cell invasion and tumorigenesis were used to obseve the effects ofHNF-1αmutation andAPCmutation on the proliferation ability of FAP cells.ResultsCompared with the control group,HNF-1αcombined withAPCmutation group showed stronger cell clonal formation,cell migration,cell invasion and tumorigenicity.ConclusionHNF-1αcombined with APC mutation promotes the proliferation of FAP cells.