摘要
背景 肠道菌群与精神分裂症之间的相关机制尚不清楚。探究急性组、缓解组精神分裂症患者肠道菌群丰度差异,以及与临床症状的相关性。方法 本研究共纳入124例参与者,收集一般资料及粪便,采用16S rRNA基因测序分析肠道微生物,由精神科医生使用阳性与阴性症状量表(PANSS)评定患者症状得分。结果 三组间一般资料无明显差异,急性组、缓解组在病程、氯丙嗪当量上无明显差异。急性组PANSS各因子评分和总分均高于缓解组(P<0.05)。16SrRNA结果示三组之间在属水平上有9种肠道菌群组成发生改变。相关性分析示,急性组存在4个菌属与临床症状存在相关性,缓解组中仅梭状芽孢杆菌属与症状呈负相关。结论 不同疾病阶段精神分裂症患者肠道菌群组成存在差异,且与临床症状相关。关键词:精神分裂症;肠道菌群;16S rRNA测序中图分类号:R593.2Study on the correlation between intestinal flora and clinical symptoms of schizophrenic patients at different disease stagesSHI Li 1, 2, 3 LI Jingwei 1, 2, 3 ZHANG Xulai 1, 2, 3, 4 (corresponding author)1. Affiliated Psychological Hospital of Anhui Medical University, Anhui Hefei 2300222. Anhui Provincial Mental Health Center, Anhui Hefei 2300223. Hefei Fourth People's Hospital, Anhui Hefei 2300224. Anhui Provincial Clinical Medical Research Center for Mental and Psychological Diseases, Anhui Hefei 230022Abstract: Background The relevant mechanism between intestinal flora and schizophrenia is still unclear. Objective To explore the difference of intestinal microflora abundance between acute and remission schizophrenia patients and its correlation with clinical symptoms. Methods A total of 124 participants were included in this study. General data and faeces were collected. 16S rRNA gene sequencing was used to analyze intestinal microorganisms. Psychiatrists used the Positive and Negative Symptom Scale (PANSS) to evaluate the symptoms of patients. Results (1) There was no significant difference in the general data among the three groups. There was no significant difference in the course of disease and chlorpromazine equivalent between the acute group and the remission group. The scores of all factors and total scores of PANSS in acute group were higher than those in remission group (P<0.05). 16SrRNA results showed that the composition of 9 intestinal microflora changed at the genus level among the three groups. Correlation analysis showed that there were 4 genera of bacteria in the acute group that were correlated with clinical symptoms, and only Clostridium in the remission group was negatively correlated with symptoms. Conclusion The composition of intestinal flora in schizophrenic patients at different disease stages is different, and is related to clinical symptoms.Keywords: schizophrenia; Intestinal flora; 16S rRNA sequencing中文科技期刊数据库(全文版)医药卫生570 引言精神分裂症(SCZ)是一种严重的神经精神疾病,在全世界普通人群中流行约1%[1];现在人们普遍承认,其病因似乎在于大脑发育的复杂遗传机制和环境紊乱因素[2],而且该疾病的生物机制是异质的,相对不确定[3-5]。近年来,大量研究强调了肠道微生物群在严重精神疾病中的因果作用,包括抑郁症[6]、阿尔茨海默病[7]、双相情感障碍[8]和SCZ[9]。这些研究结果显示,微生物群可以通过肠脑轴与大脑交流。肠道微生物可以通过神经-免疫-内分泌介质扰乱情绪和大脑功能,反之亦然,中枢神经系统可以通过分泌不同的生化物质来调节肠道功能[10],如多巴胺、5-羟色胺[11]。迄今为止,大量证据表明,肠脑轴和肠道微生物组的变化可能有助于SCZ的病理生理学和临床症状的发展[12, 13];然而,由于这些证据尚不明确,两者之间的明确关系在很大程度上仍然难以捉摸,因此迫切需要确定这种疾病的精确机制。因此,本研究旨在通过16S rRNA基因测序研究SCZ不同阶段患者和健康受试者的粪便微生物区系的组成。本研究将集中在研究SCZ急性期和缓解期之间的肠道菌群差异,以及与临床症状的关联。
