蒿甲醚对大鼠肺泡Ⅱ型上皮细胞DNA的影响

Effect of artemether on DNA of type Ⅱ alveolar epithelial cells in rats

目的:蒿甲醚除了具有良好的临床治疗作用外,对正常组织有极大的副作用。实验拟观察蒿甲醚对大鼠肺泡Ⅱ型上皮细胞的影响。方法:实验于2005-09-20/2007-03-10在山东省泰山医学院生命科学研究所、中心实验室和机能实验室完成。①实验材料:蒿甲醚购自昆明制药厂,分析纯,使用前先进行预处理,处理方法:研磨后用溶液配成1g/L的母液,超声助溶解后供实验用。Wistar大鼠70只,体质量(70±15)g,雌雄不拘,由山东大学医学院实验动物中心提供。实验过程中对动物处置符合动物伦理学标准。②实验方法:将大鼠随机分为普通饲料对照组(n=10)和实验组(n=60),实验组分6个亚组,分别在饲料中加入0.1,0.2,0.4,0.8,1.6,3.2mg/kg的蒿甲醚。采用单细胞凝胶电泳技术观察大鼠肺泡Ⅱ型上皮细胞DNA的改变。结果:70只大鼠,饲养过程中实验组死亡6只,死亡原因为饲料添加蒿甲醚造成。当饲料中的蒿甲醚浓度为0.1mg/kg时,大鼠肺泡Ⅱ型上皮细胞DNA损伤率与对照组相比,差异无统计学意义;饲料中的蒿甲醚浓度超过0.2mg/kg时,大鼠肺泡Ⅱ型上皮细胞DNA损伤率明显增加,与对照组相比,差异有统计学意义。且损伤程度与饲料中蒿甲醚含量呈正相关。结论:当饲料中的蒿甲醚浓度超过0.2mg/kg时,蒿甲醚可在一定程度上改变肺泡Ⅱ型上皮细胞的DNA生物学特性。且随着蒿甲醚浓度的升高,这种损害更为明显。

AIM: Though artemether has many cure effects, it has side effects. This study was aimed to elucidate the influence of artemether on type Ⅱ alveolar epithelial cells of the rats. METHODS: The experiment was conducted between September 20th, 2005 and March 10th, 2007 in the Biological Laboratory, Central Laboratory and Mechanism Laboratory of Taishan Medical College. ①Artemether was made in Kunming Pharmaceutical Corporation. Pretreatment was performed before using analytical pure. After grinding, 1 g/L stock solution was prepared and used in experiment after dissolving by ultrasound. A total of 70 Wistar rats weighting (70±15) g, of either sex, were offered by Experimental Animal Center of Medical College of Shandong University. Animal intervention was accorded with animal ethical standard. ②These rats were randomly divided into control group (n =10) and experimental group (n =60). The experimental group was subdivided into 6 groups, and 0.1,0.2,0.4,0.8,1.6, 3.2 mg/kg artemether were added in the feed, respectively. By single cell gel electrophoresis technique, the action of artemether on DNA damage in type Ⅱ alveolar epithelial cells was observed. RESULTS: In the experimental process, 6 of the 70 rats died because fed with artemether fodder. Compared with the control group, there was no DNA changes had been observed in 0.1 mg/kg artemether fodder group. However, in over 0.2 mg/kg artemether fodder group, DNA damage of the type Ⅱ alveolar epithelial cells could be detected clearly, and the difference was significant as compared with the control group. The damage was positively correlated with the content of artemether. CONCLUSION: If over 0.2 mg/kg, artemether can change the biological feature of DNA of the type Ⅱ alveolar epithelial cells to some extent. With the increasing content of artemether, the degree of the DNA damage will be more seriously.